Comparable functions of plasmacytoid and monocyte-derived dendritic cells in chronic hepatitis C patients and healthy donors

Background/Aims

Dendritic cells (DCs) play a key role in immune responses through antigen presentation and cytokine secretion. Hepatitis C virus (HCV) is able to escape elimination by the immune system and often establishes a chronic infection. To investigate whether DC dysfunction is involved in this process, we have studied monoycte-derived DCs (Mo-DCs) and plasmacytoid DCs (pDCs), which produce large amounts of IFN-α, from chronic HCV patients and healthy donors.

Methods

We have assessed TNF-α and IFN-α production by pDCs using intracellular staining after total PBMCs stimulation with unmethylated CG dinucleotides (CpGs). The induction of allogeneic T cell proliferation by immature Mo-DCs was measured using the MLR assay. The up-regulation of maturation markers and the production of TNF-α in response to LPS were analyzed using flow cytometry and ELISA, respectively.

Results

We have detected comparable frequencies of pDCs producing TNF-α and IFN-α in both chronic HCV patients and healthy donors and we have found that immature Mo-DCs from both patients and donors similarly induce allogeneic T cell proliferation and mature and secrete TNF-α in response to LPS.

Conclusions

Our results demonstrate that both pDC and Mo-DCs are not impaired in HCV infected patients.

Keywords: Dendritic cell, HCV, Maturation, TNF-α, IFN-α